Insufficiently degassed mobile phase in HPLC will lead to higher baseline noise, spikes due to air bubbles, and significant drifting and oscillation of the detector signal in HPLC. These phenomena may lead to significant difficulties in integration of peaks and create problems for quantitative analyses. Dissolved gases affect the operation of the pump and may cause strong pulsation and flow rate changes. This results in serious retention time shifts, making analyte identification difficult.
The
SunChrom Degasser eliminates these problems through the efficient degassing of the eluent. Vacuum degassing is an extremely efficient method of degassing mobile phase and provides a number of benefits when compared to the use of ultrasonic and helium degassing.
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Vacuum degassing is one of the most efficient methods for degassing eluents. The degasser is placed between the eluent reservoir and the solvent delivery pump. The eluent is drawn through a PTFE tube (Teflon®) that is located in a vacuum chamber. Semi-permeable PTFE tube allows small gaseous molecules to diffuse through the tubing, but the eluent molecules are unable to do so. After the eluent has moved through the PTFE tube, the gas concentration will be reduced down to < 1 ppm.
The new generation of degasser have only one semi-permeable Teflon® AF tube. This material is hundredfold more permeable to gaseous substances than “simple” Teflon® and is more hydrophilic. Due to the very small surface area, biological contamination is minimal and cleaning the tubing is simplified. To rinse the degasser completely or to change an eluent you now need only 1-2 mL instead of 25-40 mL. The internal vacuum pump is also maintenance-free for 5 years.
Cross contamination between eluents is eliminated because of individual vacuum chambers for each eluate.
The
SunChrom vacuum degasser is available with two or four channels, so that you can degass up to four eluents at the same time with a single system.
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